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rabbit anti hif 2a  (Proteintech)


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    Proteintech rabbit anti hif 2a
    Rabbit Anti Hif 2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+hif+2a/EPAS1+Antibody/pm41241842-358-41-74
    Average 94 stars, based on 48 article reviews
    rabbit anti hif 2a - by Bioz Stars, 2026-09
    94/100 stars

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    Western Blot:

    Article Title: Oxygen-independent expression of HIF-1α during the cell cycle in hepatocellular carcinoma cells controls essential metabolic pathways under normoxia.
    Article Snippet: .. Antibody References Working dilution Primary antibodies Mouse anti-b-actin 3700, Cell Signaling, USA WB 1:5000 Mouse anti-a-tubulin 3873, Cell Signaling, USA WB 1:10000 Mouse anti-HIF-1a 610 959, BD Biosciences, USA WB 1:500 Rabbit anti-HIF-1a [60] WB 1:2000; IP: 2 lg mL 1 Rabbit anti-HIF-2a [61] WB 1:500 Rabbit anti-ΗΚΙΙ 2867, Cell Signaling, USA WB 1:1000 Rabbit anti-EGFR 2232, Cell Signaling, USA WB 1:1000 Rabbit anti-GAPDH 5174, Cell Signaling, USA WB 1:1000 Rabbit anti-CUL2 10 981-2-AP, Proteintech, Manchester, UK WB 1:3000 Rabbit anti-CyclinB1 Sc-752, Santa Cruz Biotechnology, Dallas, TX, USA WB 1:250 Goat anti-GFP SICGEN, Cantanhede, Portugal WB 1:5000 Mouse anti-ubiquitin Sc-8017, Santa Cruz Biotechnology, Dallas, TX, USA WB 1:250 Rabbit anti-hydroxyproline 73 812, Cell Signaling, USA WB 1:500 Mouse anti-Cdk1 33–1800, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA WB 1:1000 Goat anti-MCM3 Sc9849, Santa Cruz Biotechnology, Dallas, TX, USA WB 1:250 Normal rabbit IgG 2729, Cell Signaling, USA IP: 2 lg mL 1 Secondary antibodies Goat anti-rabbit HRP AP187P, Sigma-Aldrich, Inc, USA WB 1:5000 Horse anti-mouse HRP 7076, Cell Signaling, USA WB 1:5000 Mouse anti-goat HRP Sc-2354, Santa Cruz Biotechnology, Dallas, TX, USA WB 1:2000 22 a 2025 The Author(s). ..



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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) <t>HIF-1a</t> and <t>HIF-2a</t> expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.
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    Image Search Results


    The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) HIF-1a and HIF-2a expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.

    Journal: Cancers

    Article Title: 5-Aminolevulinic Acid: A Novel Approach to Improving Radioresistance in Prostate Cancer

    doi: 10.3390/cancers17081286

    Figure Lengend Snippet: The viability of parental PCa cells and the established radioresistant PCa cells 24 h after 5-ALA administration (( a ) PC-3 and PC-3-R; ( b ) DU 145 and DU 145-R). The survival curves of the relative survival fractions in the parental PCa cells and the established radioresistant PCa cells after IR (2–8 Gy single dose) alone or a combination of 1 mM 5-ALA and IR (( c ) PC-3 and PC-3-R; ( d ) DU 145 and DU 145-R). ( e , f ) HIF-1a and HIF-2a expression in the parental and radioresistant PCa cells was measured via Western blot analysis. 5-ALA: 5-aminolevurinic acid. D 50% : radiation dose required to achieve a 50% survival rate. HIF: hypoxia-inducible factor. IC 50 : half-maximal inhibitory concentration. IR: irradiation. PCa: prostate cancer. * p < 0.05, *** p < 0.001. Original western blots are presented in File S1.

    Article Snippet: The primary antibodies used included anti-β-actin rabbit polyclonal antibody (cat. no. 20536-1-AP; dilution 1:2000; Proteintech, Rosemont, IL, USA), anti-hypoxia-inducible factor 1a (HIF-1a) rabbit polyclonal antibody (cat. No. R12-2180; dilution 1:500; Assay Biotechnology, Fremont, CA, USA), anti-HIF-2a rabbit polyclonal antibody (cat. no. NB100-122; dilution 1:500; Novus Biologicals, Centennial, CO, USA), anti-ATP-binding cassette transporter subfamily G2 (ABCG2) rabbit polyclonal antibody (cat. no. 27286-1-AP; dilution 1:1000; Proteintech), anti-ferrochelatase murine monoclonal antibody (cat. no. sc-377377; dilution 1:500; Santa Cruz Biotechnology, Dallas, TX, USA), anti-proton-coupled peptide transporter 1 (PEPT-1) rabbit polyclonal antibody (cat. no. sc-20653; dilution 1:200; Proteintech), anti-B-cell/CLL lymphoma 2 (BCL-2) murine monoclonal antibody (cat. no. sc-7382; dilution, 1:200; Santa Cruz Bio-technology), anti-BCL-2-associated X protein (BAX) rabbit polyclonal antibody (cat. no. sc-526; dilution, 1:200; Santa Cruz Biotechnology), anti-BCL-2-associated agonist of cell death (BAD) murine monoclonal antibody (cat. no. sc-8044; dilution 1:200; Santa Cruz Biotechnology), and anti-BCL extra-large (BCL-xL) murine monoclonal antibody (cat. no. sc-8392; dilution 1:200; Santa Cruz Biotechnology).

    Techniques: Expressing, Western Blot, Concentration Assay, Irradiation